Anti-AADACL1/NCEH1 Antibody Picoband™

Product Name

Anti-AADACL1/NCEH1 Antibody Picoband™

View all AADACL1 Antibodies

SKU/Catalog Number

A06478-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-AADACL1/NCEH1 Antibody Picoband™ catalog # A06478-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-AADACL1/NCEH1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06478-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human AADACL1/NCEH1 recombinant protein (Position: Q44-D352).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06478-1 is reactive to NCEH1 in Human

Applications

A06478-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

45.808kDa

Background of AADACL1

Neutral cholesterol ester hydrolase 1 (NCEH) also known as arylacetamide deacetylase-like 1 (AADACL1) or KIAA1363 is an enzyme that in humans is encoded by the NCEH1 gene. Predicted to enable hydrolase activity. Predicted to be involved in ether lipid metabolic process. Predicted to act upstream of or within SMAD protein signal transduction; protein dephosphorylation; and xenobiotic metabolic process. Located in membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of AADACL1/NCEH1 using anti-AADACL1/NCEH1 antibody (A06478-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AADACL1/NCEH1 antigen affinity purified polyclonal antibody (Catalog # A06478-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AADACL1/NCEH1 at approximately 46 kDa. The expected band size for AADACL1/NCEH1 is at 46,55 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-AADACL1/NCEH1 antibody (A06478-1).
Overlay histogram showing HepG2 cells stained with A06478-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AADACL1/NCEH1 Antibody (A06478-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NCEH1 (Source: Uniprot.org, NCBI)

Alternative Names

AADACL1; EC 3.1.1.79; KIAA1363EC 3.1.1; NCEHArylacetamide deacetylase-like 1EC 3.1.1.-; neutral cholesterol ester hydrolase 1 NCEH1 AADACL1, NCEH neutral cholesterol ester hydrolase 1 neutral cholesterol ester hydrolase 1|acetylalkylglycerol acetylhydrolase|alkylacetylglycerol acetylhydrolase|arylacetamide deacetylase-like 1

For more info on NCEH1, check out the NCEH1 Infographic

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In this infographic, you will see the following information for NCEH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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